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(A) Quantitative RT-PCR of ileal tissue from C57Bl/6 WT mice exposed to sham or burn injury, with or without ethanol exposure. (B-E) Quantitative RT-PCR of ileal tissue from Reg3g-Tg or littermate control mice exposed to sham or burn ethanol. (B) REG3G (C) REG3B (D) Defensin, alpha, related sequence 1 <t>(Defa-rs1)</t> and (E) Beta defensin 3 (Defb3). Data shown as mean values ± SEM (dots represent individual animals). * p < 0.05 compared to all other groups, ^ p < 0.05 compared to sham groups, # p < 0.05 compared to both burn groups (one-way ANOVA with Tukey’s multiple comparison test). All experiments represent 8-10 mice per group.
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(A) Quantitative RT-PCR of ileal tissue from C57Bl/6 WT mice exposed to sham or burn injury, with or without ethanol exposure. (B-E) Quantitative RT-PCR of ileal tissue from Reg3g-Tg or littermate control mice exposed to sham or burn ethanol. (B) REG3G (C) REG3B (D) Defensin, alpha, related sequence 1 <t>(Defa-rs1)</t> and (E) Beta defensin 3 (Defb3). Data shown as mean values ± SEM (dots represent individual animals). * p < 0.05 compared to all other groups, ^ p < 0.05 compared to sham groups, # p < 0.05 compared to both burn groups (one-way ANOVA with Tukey’s multiple comparison test). All experiments represent 8-10 mice per group.
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(A) Quantitative RT-PCR of ileal tissue from C57Bl/6 WT mice exposed to sham or burn injury, with or without ethanol exposure. (B-E) Quantitative RT-PCR of ileal tissue from Reg3g-Tg or littermate control mice exposed to sham or burn ethanol. (B) REG3G (C) REG3B (D) Defensin, alpha, related sequence 1 <t>(Defa-rs1)</t> and (E) Beta defensin 3 (Defb3). Data shown as mean values ± SEM (dots represent individual animals). * p < 0.05 compared to all other groups, ^ p < 0.05 compared to sham groups, # p < 0.05 compared to both burn groups (one-way ANOVA with Tukey’s multiple comparison test). All experiments represent 8-10 mice per group.
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(A) Quantitative RT-PCR of ileal tissue from C57Bl/6 WT mice exposed to sham or burn injury, with or without ethanol exposure. (B-E) Quantitative RT-PCR of ileal tissue from Reg3g-Tg or littermate control mice exposed to sham or burn ethanol. (B) REG3G (C) REG3B (D) Defensin, alpha, related sequence 1 <t>(Defa-rs1)</t> and (E) Beta defensin 3 (Defb3). Data shown as mean values ± SEM (dots represent individual animals). * p < 0.05 compared to all other groups, ^ p < 0.05 compared to sham groups, # p < 0.05 compared to both burn groups (one-way ANOVA with Tukey’s multiple comparison test). All experiments represent 8-10 mice per group.
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(A) Quantitative RT-PCR of ileal tissue from C57Bl/6 WT mice exposed to sham or burn injury, with or without ethanol exposure. (B-E) Quantitative RT-PCR of ileal tissue from Reg3g-Tg or littermate control mice exposed to sham or burn ethanol. (B) REG3G (C) REG3B (D) Defensin, alpha, related sequence 1 <t>(Defa-rs1)</t> and (E) Beta defensin 3 (Defb3). Data shown as mean values ± SEM (dots represent individual animals). * p < 0.05 compared to all other groups, ^ p < 0.05 compared to sham groups, # p < 0.05 compared to both burn groups (one-way ANOVA with Tukey’s multiple comparison test). All experiments represent 8-10 mice per group.
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Expression of <t>retinoschisin</t> in mutant and wild-type retinas. In wild-type retinas, retinoschisin <t>(RS1;</t> ( Aa )) and β2 ( Ab ) were co-localized ( Ac ) in the photoreceptor inner segments, outer plexiform layer, and cell bodies in the inner nuclear layer. In β2 / β1 ki retinas ( Ae – Ag ), retinoschisin was barely detectable and diffusely distributed ( Ae ). β2 was not detected in mutant retinas as expected ( Af , Ag ) is the overlay of ( Ae , Af ). As a negative control, sections were incubated with the secondary antibodies only ( Ad , Ah ). ( B ) Western blot analysis confirmed massively reduced levels of retinoschisin in mutant retinas when compared to wild-type retinas. Note that three-fold more mutant than wild-type samples were loaded. gcl: ganglion cell layer; inl: inner nuclear layer; ki: knock-in; onl: outer nuclear layer; RS1: retinoschisin; TPS: total protein stain; wt: wild-type. Scale bar: 50 µm.
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(A) Quantitative RT-PCR of ileal tissue from C57Bl/6 WT mice exposed to sham or burn injury, with or without ethanol exposure. (B-E) Quantitative RT-PCR of ileal tissue from Reg3g-Tg or littermate control mice exposed to sham or burn ethanol. (B) REG3G (C) REG3B (D) Defensin, alpha, related sequence 1 (Defa-rs1) and (E) Beta defensin 3 (Defb3). Data shown as mean values ± SEM (dots represent individual animals). * p < 0.05 compared to all other groups, ^ p < 0.05 compared to sham groups, # p < 0.05 compared to both burn groups (one-way ANOVA with Tukey’s multiple comparison test). All experiments represent 8-10 mice per group.

Journal: Shock (Augusta, Ga.)

Article Title: Intestinal REG3G Protects Against Gastrointestinal Dysfunction in a Murine Model of Ethanol Intoxication and Burn Injury

doi: 10.1097/SHK.0000000000002737

Figure Lengend Snippet: (A) Quantitative RT-PCR of ileal tissue from C57Bl/6 WT mice exposed to sham or burn injury, with or without ethanol exposure. (B-E) Quantitative RT-PCR of ileal tissue from Reg3g-Tg or littermate control mice exposed to sham or burn ethanol. (B) REG3G (C) REG3B (D) Defensin, alpha, related sequence 1 (Defa-rs1) and (E) Beta defensin 3 (Defb3). Data shown as mean values ± SEM (dots represent individual animals). * p < 0.05 compared to all other groups, ^ p < 0.05 compared to sham groups, # p < 0.05 compared to both burn groups (one-way ANOVA with Tukey’s multiple comparison test). All experiments represent 8-10 mice per group.

Article Snippet: RT-PCR was performed on QuantStudio 3 Real-Time PCR System (Thermo Fisher) using TaqMan probes and reagents: cxcl1 (Mm04207460_m1), Defa-rs1 (Mm00655850_m1), defb3 (Mm04214158_s1), il6 (Mm00446190_m1), Reg3g (Mm00441127-m1), Reg3b (Mm00440616_g1), s100a8 (Mm00496696_g1), Tnfa (Mm00443258_m1), Il1b (Mm00434228_m1), Tjp1 (Mm01320638_m1), Cdh1 (Mm01247357_m1, Cldn1 (Mm01342184_m1), ccl2 (Mm00441242_m1), Saa1 (Mm00656927_g1).

Techniques: Expressing, Quantitative RT-PCR, Control, Sequencing, Comparison

Expression of retinoschisin in mutant and wild-type retinas. In wild-type retinas, retinoschisin (RS1; ( Aa )) and β2 ( Ab ) were co-localized ( Ac ) in the photoreceptor inner segments, outer plexiform layer, and cell bodies in the inner nuclear layer. In β2 / β1 ki retinas ( Ae – Ag ), retinoschisin was barely detectable and diffusely distributed ( Ae ). β2 was not detected in mutant retinas as expected ( Af , Ag ) is the overlay of ( Ae , Af ). As a negative control, sections were incubated with the secondary antibodies only ( Ad , Ah ). ( B ) Western blot analysis confirmed massively reduced levels of retinoschisin in mutant retinas when compared to wild-type retinas. Note that three-fold more mutant than wild-type samples were loaded. gcl: ganglion cell layer; inl: inner nuclear layer; ki: knock-in; onl: outer nuclear layer; RS1: retinoschisin; TPS: total protein stain; wt: wild-type. Scale bar: 50 µm.

Journal: Cells

Article Title: Atp1b2 Atp1b1 Knock-In Mice Exhibit a Cone–Rod Dystrophy-Like Phenotype

doi: 10.3390/cells14120878

Figure Lengend Snippet: Expression of retinoschisin in mutant and wild-type retinas. In wild-type retinas, retinoschisin (RS1; ( Aa )) and β2 ( Ab ) were co-localized ( Ac ) in the photoreceptor inner segments, outer plexiform layer, and cell bodies in the inner nuclear layer. In β2 / β1 ki retinas ( Ae – Ag ), retinoschisin was barely detectable and diffusely distributed ( Ae ). β2 was not detected in mutant retinas as expected ( Af , Ag ) is the overlay of ( Ae , Af ). As a negative control, sections were incubated with the secondary antibodies only ( Ad , Ah ). ( B ) Western blot analysis confirmed massively reduced levels of retinoschisin in mutant retinas when compared to wild-type retinas. Note that three-fold more mutant than wild-type samples were loaded. gcl: ganglion cell layer; inl: inner nuclear layer; ki: knock-in; onl: outer nuclear layer; RS1: retinoschisin; TPS: total protein stain; wt: wild-type. Scale bar: 50 µm.

Article Snippet: retinoschisin (RS1) , 1:500 (IHC) 1:500 (WB) , Proteintech Group, Inc., Rosemont, IL, USA , 24430-1-AP.

Techniques: Expressing, Mutagenesis, Negative Control, Incubation, Western Blot, Knock-In, Staining